The LAL reagent is formulated with a synthetic substrate which produces a chromophore when cleaved by endotoxin activated enzyme. The test is read at 405 nm, usually in a microplate reader. The maximum sensitivity of 0.001 EU/mL can be achieved when using ACC's Pyrochrome® Chromogenic reagent with Glucashield® Buffer.

• Requires a tube reader or incubating microplate reader (an incubating reader is required for the kinetic method)
• Maximum sensitivity to 0.001 EU/mL when using ACC's Pyrochrome® reagent with Glucashield® Buffer
• A quantitative assay providing electronic stored data and print-outs of results
• Incubation time varies depending on the standard curve range
• Higher sensitivity allows for greater dilution to overcome interference
• The option of a diazo-coupled endpoint method (read at 540–550 nm) is available, which is useful for samples that absorb light at 405 nm